Genetics Lab photos

        

    Examination of fetal cells in culture   

 

  

  Analysis on a computerised microscope

 

           

             Normal chromosomes  

 

  

    Breast cancer (fluorescence in situ) 

 

          

                  DNA sample 

 

 

       Multiple sample electrophoresis

 

 

Comparative Genomic Hybridisation

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Welcome to the HRSRH Genetics Laboratory

As part of the Northern Regional Genetics Program, our mandate is the provision and facilitation of Genetics Laboratory Services throughout western and eastern Northern Ontario.

Services
  • The techniques used include cell culture, metaphase analysis, karyotyping, G-banding, C-banding, Nucleolar Organiser Region (NOR) staining, Fluorescence In Situ Hybridization (FISH) of metaphases and interphase nuclei with molecular DNA probes, as well as Comparative Genomic Hybridization (CGH) with microarray, for congenital and acquired chromosomal abnormalities.
  • The samples processed include blood, amniotic fluid, fibroblasts, bone marrow, tumours and paraffin embedded tissues.
  • The cytogenetics laboratory services are offered for the investigation of:
    • Prenatal diagnosis 
    • Congenital abnormalities 
    • Developmental delay 
    • Genetic syndromes 
    • Familial chromosomal rearrangements 
    • Reproductive problems 
    • Infertility 
    • Non-inherited cancer
  • Other specialised biochemical and molecular laboratory services are referred out and forwarded to the appropriate provincial laboratories providing these services.
  • For a copy of our Chromosomal Analysis Requisition Forms, click here (clinical) and here (cancer).
  • For microarray CGH tests done for congenital studies, patients must consent to, or opt-out from, sharing their anonymised data with international genomic databases.  Click here for consent form.
Instructions for Sample Collection

For all samples:

  1. Inform the laboratory by telephone that a sample is being sent. This ensures that a trace can be made to determine the location of lost or delayed samples. 
  2. Label tube with patient’s name and lab number. Ensure that the requisition form is completed with patient’s name, date of birth, address, health card number, relevant clinical information, test required and referring physician’s name and address. 
  3. Forward to our lab as soon as possible at room temperature. Do not freeze. Keep away from heat.

Peripheral blood: 

  1. No fasting required 
  2. Collect unclotted blood in a sterile, sodium heparin vacutainer (for karyotype and/or FISH studies) and/or in EDTA (for microarray CGH investigations): 
  3. 5-8 cc for adults, 1-2 cc for babies

Bone marrow: Collect 1-2 cc of unclotted bone marrow in a sterile, sodium heparin vacutainer (for karyotype and/or FISH studies) and in EDTA (for possible microarray CGH investigations):

Amniotic fluid: Collect 18-20 cc of fluid in a sterile container.

Skin: Inner forearm or shoulder blade are preferred. Clean the area with alcohol and let dry completely. A 3x2x0.5 mm pinch biopsy is enough. Pinpoint bleeding at the pinch assures that live fibroblasts are included in the biopsy. Put in a tube or small bottle completely filled with culture medium or Hank’s balanced salt solution or sterile normal saline solution. Indicate the site of biopsy on the label.

Products of conception: Take at least two separate sterile samples not exceeding 5x5x5 mm from different locations including skin. Put in a tube or small bottle completely filled with culture medium or Hank’s balanced salt solution or sterile normal saline solution. Indicate the site of each sample on each label.

Tumours and lymph nodes: Put tumour sample (not exceeding 5x5x5 mm) and up to the entire lymph node in a tube or bottle, completely filled with culture medium or Hank’s balanced salt solution or sterile normal saline solution. Indicate the site of origin on the label.

Paraffin embedded tissue for FISH study: Use formalin fixed samples only. Put a 4μm thin slice on an ionised slide, with smaller negative and positive control samples at the end of the same slide. Clearly indicate which sample is which, and which area of the patient’s sample needs testing (make drawing or use marker on accompanying H&E slide that we will return to your lab).

Contact Us

The Genetics Laboratory is not open to the public. Test results are only given to referring health professionals. The laboratory is located in the South Tower of the Sudbury Regional Hospital (41 Ramsey Lake Road, Sudbury ON P3E 5J1).

Referring centres and health professionals can contact the laboratory at:

Telephone: (705) 675-4782
Fax: (705) 675-4776

The Cytogenetics laboratory is open from 7:30 a.m. to 3:30 p.m. from Monday to Friday. 


Administrative Director:
Ms. Lorena Cabral, MBA, BSc, MRT(R), CHE

Scientific Director:
Dr. Gilbert B. Côté, PhD, FCCMG

Lab Managers:
Mr. Rick Mancini
Ms. Pam Kirwan

Lab Supervisors:
Ms. Theresa Paradis
Ms. Loreen Veno
Ms. Lorna Nawalaniec

Senior technologist:
Ms. Anne McBain, HBSc, MLT (CG)

Medical technologists:
Ms. Victoria Creighton, HBSc, MLT (CG)
Ms. Alexandra Noussi, MLT(CG)
Ms. Amanda Piche-Marolt, HBSc, MLT (G)
Mr. Steve Reid, BSc, MLT(G)
Ms. Shabnam Salehi-Rad, HBSC, MLT(G)
Ms. Bernice Suitor, HBSc, MLT (CG)

Secretary:
Ms. Paula Haas